Target Name: Human CD64, FCG1, FCGR1, FCGR1A, IGFR1
Source: Human CD64 protein (accession number P12314) (Gln16-Thr287) with C-terminus His tag and Avi tag is expressed in CHO cells. This protein was site-specifically labeled with Biotin by BirA ligase.
Molecular Weight: The 307 amino acid protein has a predicted molecular weight of 34.2kDa. The protein migrates at approximately 50-60 kDa on SDS-PAGE with DTT-reduced conditions.
Purity: >95% based on SDS-PAGE
Formulation: 1xPBS buffer, 0.22 µm filtered
Vial Size: 25µg, 100µg
Protein Concentration: 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling: Quick spin the vial after receiving. An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. The protein can be further diluted to 0.1 mg/mL with 0.22 µm filtered buffer. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles. Carrier proteins such as FBS and BSA need to be added if further dilution is needed.
Biotinylated human CD64 (C-His-Avi) Protein