Human PD-L1; Programmed Cell Death 1 Ligand 1; HumanB7-H Protein
Recombinant Human PD-L1 (Phe19-Thr239) with C-terminus Fc tag & Avi tag is expressed in HEK293 cells. This protein was site-specifically labeled with Biotin by BirA ligase.
The protein has a predicted molecular weight of 54kDa. The protein migrates at approximately 65-70kDa on SDS-PAGE with DTT-reduced conditions.
>95% based on SDS-PAGE.
1xPBS, pH7.4, 0.22 µm filtered.
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot specific concentration
Storage and Handling:
Quick spin the vial after receiving. An unopened vial can be stored at 2-8°C for 2 weeks or at -20°C and below for six months. The protein can be further diluted to 0.1 mg/mL with 0.22 µm filtered Tris PH 7.5 buffer. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles. Carrier proteins such as BSA need to be added if further dilution is needed.
Biotinylated Human PD-L1 Protein (C-Fc-Avi)