Target Name: 
Human CD279 Protein, Human PD-1 Protein
Source:
Human PD-1 protein (NP_005009.2) (Leu25-Gln167) with C-terminus His tag-Avi tag is expressed in HEK293 cells. This protein was site-specifically labeled with Biotin by BirA ligase. The labeling efficiency is greater than 90% by gel shift assay.
Molecular Weight:
The 176 amino acid protein has the predicted molecular weight of 19.5 kDa. The protein migrates at approximately 28-45 kDa on SDS-PAGE with DTT-reduced condition.
Purity:
>95% based on SDS-PAGE
Formulation:
1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin Level:
Not Tested
Vial Size:
25µg, 100µg
Protein Concentration:
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling: 
Quick spin the vial after receiving. Unopened vial can be stored at 2-8°C for 2 weeks or at -20°C and below for six months. The protein can be further diluted to 0.1 mg/mL with 0.22 µm filtered PBS buffer. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles. Carrier protein such as BSA need to be added if further dilution is needed.