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Target Name: SARS-CoV2 Nucleocpasid Protein, NP

Source: SARS-CoV2 Nucleocapsid Protein (accession number QHD43423.2) (Met1-Ala419) with C-terminus 6His tag is expressed in HEK293 cells. This protein is Biotinylated using a chemical reaction.

Molecular Weight: The 425 amino acid protein has a predicted molecular weight of 46.4 kDa. The protein migrates at approximately 50 kDa on SDS-PAGE with DTT-reduced condition.

Purity: >95% based on SDS-PAGE

Formulation: 1xPBS, 0.22 µm filtered

Vial Size: 25µg, 100µg

Protein Concentration: 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot specific concentration.

Storage and Handling: Quick spin the vial after receiving. An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. The protein can be further diluted to 0.1 mg/mL with 0.22 µm filtered buffer.  This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles. Carrier proteins such as FBS and BSA need to be added if further dilution is needed.

Biotinylated SARS-CoV-2 Nucleocapsid Protein (C-His)

  • HEK 293

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