Target Name: SARS-CoV2 Spike S1 Protein, S1 Protein
Source: SARS-CoV-2 S protein (accession number QHD43416.1) S1 domain (Val16-Arg685) with D614G mutation, and with C-terminus His-Avi tag is expressed in CHO cells. This protein is biotinylated in vitro by BirA ligase. The efficiency of biotinylation is greater than 90% by gel shift assay.
Molecular Weight: The 706 amino acid protein has a predicted molecular weight of 78.8 kDa. The protein migrates at approximately 90-120 kDa on SDS-PAGE with DTT-reduced conditions.
Purity: >90% based on SDS-PAGE
Formulation: PBS, 0.22 µm filtered
Vial Size: 25µg, 100µg
Protein Concentration: 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling: Quick spin the vial after receiving. An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. The protein can be further diluted to 0.1 mg/mL with 0.22 µm filtered PBS. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles. Carrier proteins such as FBS and BSA need to be added if further dilution is needed.
Biotinylated SARS-CoV-2 Spike S1 Protein (D614G) (C-His-Avi)