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1. Kit component: 100 test

  • Annexin V-FITC:0.5mL
  • Propidium Iodide:0.5mL
  • 1 x Binding buffer: 50mL

2. Protocol:

(1) Wash cells with cold 1xPBS twice and resuspend cells in binding buffer at 1x10^6 cells/mL 

(2) Aliquot 100 μl of cells to a microcentrifuge tube (~1x10^5 total cells). 

(3) Add 5µL of Annexin V- FITC 

(4) Add 5μL of  Propidium Iodide (PI)

(5) Gently vortex (3-4 setting) the cells and incubate for 15 minutes at room temperature (25°C), protected from light.

(6) Add 390µL of Annexin V Binding Buffer.

(7) Analyze by flow cytometry.


FITC Annexin V Apoptosis Detection Kit

SKU: 100 Test
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