Target Name: ACE2, Angiotensin I converting Enzyme 2, ACEH
Source: Human ACE2 protein (accession number Q9BYF1) (Gln18-Ser740) with N-terminus His tag is expressed in HEK293 cells
Molecular Weight: The 750 amino acid protein has a predicted molecular weight of 86.5 kDa. The protein migrates at approximately 90-110 kDa on SDS-PAGE with DTT-reduced conditions. The protein was labeled with Biotin at approximately 1 biotin/protein molecule
Purity: >95% based on SDS-PAGE
Formulation: PBS, 0.22 µm filtered
Vial Size: 25µg, 100µg
Protein Concentration: 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling: Quick spin the vial after receiving. An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. The protein can be further diluted to 0.1 mg/mL with 0.22 µm filtered PBS buffer. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles. Carrier proteins such as FBS and BSA need to be added if further dilution is needed.
Biotinylated Human ACE-2 Protein (N-His)