B-Lymphocyte Antigen CD19; B-Lymphocyte Surface Antigen B4; Differentiation Antigen CD19; T-Cell Surface Antigen Leu-12; CD19
Recombinant Human CD19 (accession number P15391) (Glu21-Lys291) with an Fc tag at the C-terminus is expressed in CHO cells.
The 503 amino acid protein has a predicted molecular weight of 56.2kDa. The protein migrates at approximately 78kDa on SDS-PAGE with DTT-reduced condition.
1xPBS buffer, pH7.4, 0.22 µm filtered
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot-specific concentration.
Storage and Handling:
Quick spin the vial after receiving. An unopened vial can be stored at 2-8°C for 2 weeks or at -20°C and below for six months. The protein can be further diluted to 0.1 mg/mL with 0.22 µm filtered PBS buffer. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles. Carrier proteins such as BSA needs to be added if further dilution is needed.
Human CD19 Protein (C-Fc)