Target Name: Human Interleukin-1 beta Protein; IL-1b; IL-1 beta; Catabolin; IL1F2; IL1B.
Source: Recombinant Human IL-1b (accession number P01584) (Ala117-Ser269) is expressed in HEK293 cells.
Molecular Weight: The 153 amino acid protein has a predicted molecular weight of 17.5 kDa. The protein migrates at approximately 17 kDa on SDS-PAGE with DTT-reduced condition.
Purity: >95% based on SDS-PAGE
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl, pH7.5.
Vial Size: 10µg, 50µg
Endotoxin Level: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage and Handling: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Recombinant Human IL-1 beta Protein