Human Mesothelin Protein; MSLN; MPF
Human Mesothelin protein (Accession # AAH09272.1) (Glu296-Gly580) with C-terminus His tag is expressed in HEK293 cells.
The 298 amino acid protein has a predicted molecular weight of 33.7 kDa. The protein migrates at approximately 35-45 kDa on SDS-PAGE with DTT-reduced conditions.
>95% based on SDS-PAGE.
1xPBS, pH7.4, 0.22 µm filtered.
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot specific
Storage and Handling:
Quick spin the vial after receiving. An unopened vial can be stored at 2-8°C for 2 weeks or at -20°C and below for six months. The protein can be further diluted to 0.1 mg/mL with 0.22 µm filtered buffer. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles. Carrier proteins such as FBS and BSA need to be added if further dilution is needed.
Human Mesothelin (E296-G580) Protein (C-His)