Target Name: Granulocyte-Macrophage Colony-Stimulating Factor; GM-CSF; Colony-Stimulating Factor; CSF; Molgramostin; Sargramostim; CSF2; GMCSF
Source: Recombinant Human GM-CSF (accession number P04141) (Ala18-Glu144) is expressed in the E.coli expression system.
Molecular Weight: The 127 amino acid protein has a predicted molecular weight of 14.6 kDa. The protein migrates at approximately 14 kDa on SDS-PAGE with DTT-reduced condition
Purity: >95% based on SDS-PAGE
Formulation: Lyophilized from a 0.2 μm filtered PBS, pH 7.4.
Vial Size: 10µg, 50µg
Endotoxin Level: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage and Handling: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.