Target Name: Interleukin-12 subunit alpha;IL-12A;Cytotoxic lymphocyte maturation factor 35 kDa subunit;CLMF p35;IL-12 subunit p35;NK cell;IL12A ;NKSF1 stimulatory factor chain 1
Source: RRecombinant Human IL-12 (accession number P29459&P29460) (Arg23-Ser219&Ile23-Ser328) is expressed in HEK293 cells.
Molecular Weight: The 503 amino acid protein has a predicted molecular weight of 22.5&34.7kDa. The heterodimer migrates at approximately 25-38 kDa and 40-50 kDa on SDS-PAGE with DTT-reduced conditions.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, PH7.4.
Vial Size: 10µg, 50µg
Endotoxin Level: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage and Handling: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.