Target Name: Interleukin-18; Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma;GIF; IL-18; IL-1g; IL1F4; MGC12320; IL18
Source: Recombinant Human IL-18 (accession number Q14116) (Tyr37-Asp193) with a 6His tag at the C-terminus is expressed in HEK293 cells.
Molecular Weight: The 112 amino acid protein has a predicted molecular weight of 19.25kDa. The protein migrates at approximately 23kDa on SDS-PAGE with DTT-reduced condition.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM phosphate buffer, 150mM NaCl, pH 7.4.
Vial Size: 10µg, 50µg
Endotoxin Level: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage and Handling: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.