Target Name: Interleukin-8; IL-8; C-X-C Motif Chemokine 8; Monocyte-Derived Neutrophil Chemotactic Factor; MDNCF; Protein 3-10C; T-Cell Chemotactic Factor; IL8; CXCL8
Source: Recombinant Human IL-8 (accession number P10145) (Ser28-Ser99) is expressed in E.coli.
Molecular Weight: The 72 amino acid protein has a predicted molecular weight of 8.45kDa. The protein migrates at approximately 10kDa on SDS-PAGE with DTT-reduced condition.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM phosphate buffer, 150mM NaCl, pH 7.4.
Vial Size: 10µg, 50µg
Endotoxin Level: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage and Handling: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.