Target Name: SARS-CoV2 Nucleocpasid Protein, NP
Source: SARS-CoV2 Nucleocapsid Protein Met1-Ala419 (accession number QHD43423.2) is produced by the E.coli expression system. This protein has a 6His tag at the N-terminus.
Molecular Weight: The 425 amino acid protein has a predicted molecular weight of 49.4 kDa. The protein migrates at approximately 50-60 kDa on SDS-PAGE with DTT-reduced conditions.
Purity: >95% based on SDS-PAGE
Formulation: 20mM Hepes, 100mM NaCl , PH8.0, 0.22 µm filtered
Vial Size: 25µg, 100µg
Protein Concentration: 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling: Quick spin the vial after receiving. An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. The protein can be further diluted to 0.1 mg/mL with 0.22 µm filtered buffer. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles. Carrier proteins such as FBS and BSA need to be added if further dilution is needed.
SARS-CoV-2Nucleocapsid Protein V2 (N-His)