Target Name: SARS-CoV2 Spike Protein, active Trimer, S-Trimer
Source: Val16-Gln1208 (Accession #QHO3416) with furin mutation, two Proline mutations, and C-Terminus trimer motif, and a 10xHis tag
Expression Host: HEK293
Molecular Weight: The 1246 amino acid protein has a predicted molecular weight of 138 kDa. The protein migrates at approximately 150-180 kDa on SDS-PAGE with DTT-reduced condition
Purity: >95% based on SDS-PAGE
Formulation: PBS, 0.22 µm filtered
Concentration: 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling: Quick spin the vial after receiving. An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. The protein can be further diluted to 0.1 mg/mL with 0.22 µm filtered PBS. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles. Carrier proteins such as FBS and BSA need to be added if further dilution is needed.
SARS-CoV-2 Spike Trimer Protein (C-His), Active